Regulatory

Part:BBa_K1994032:Design

Designed by: Egheosa Ogbomo   Group: iGEM16_Warwick   (2016-10-14)


Multiple dCas9 binding sites with weak Sigma 54 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 225
    Illegal NheI site found at 260
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 248
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 229
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

dCas9 with Pam staggered sequence and sigma 54 promoter


Source

dCas9 with Pam staggered sequence and sigma 54 promoter

References