Regulatory
Part:BBa_K1994032:Design
Designed by: Egheosa Ogbomo Group: iGEM16_Warwick (2016-10-14)
Multiple dCas9 binding sites with weak Sigma 54 promoter
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 225
Illegal NheI site found at 260 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 248
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 229
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
dCas9 with Pam staggered sequence and sigma 54 promoter
Source
dCas9 with Pam staggered sequence and sigma 54 promoter